Composite

Part:BBa_K3733027:Design

Designed by: Yuanhui Sun   Group: iGEM21_HZAU-China   (2021-10-01)


The nitrate sensor(J23115-J61100) system with EBS-PyeaR and neGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 337
    Illegal BglII site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1212
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NarX and NarL gene are overlapping genes in E.coli MG1655 genome. If you want to use NarX and NarL protein separately, please to notice the overlap.


Source

The NarXL two-component signal system and PyeaR promoter come from E.coli MG1655 genome. The extra NarL binding site comes from PyeaR.

References